111年:(醫檢)微生(2)

下列何種染色法最容易觀察到Corynebacterium diphtheriae之異染顆粒( metachromatic granules )?

AGram stain
Blactophenol cotton blue stain
CLoeffler methylene blue stain
DIndia ink stain

詳細解析

本題觀念:

Corynebacterium diphtheriae之異染顆粒(metachromatic granules)為多聚磷酸(polyphosphate)顆粒,常見於菌體兩極。這些顆粒需以特殊染色法使其強烈攝取染劑並與一般菌體形成對比,才能在光學顯微鏡下清晰觀察。Loeffler’s methylene blue stain最常被用於直接在Loeffler’s medium上取樣染色,以顯示granule的深色沉澱(biocyclopedia.com)

選項分析

  • 選項A Gram stain
    標準Gram染色能呈現C. diphtheriae為Gram-positive pleomorphic rods及其“Chinese letter”排列,但metachromatic granules無法特異攝取Crystal Violet或Safranin,顆粒不明顯,難以鑑定([clinicalpub.com](https://clinicalpub.com/corynebacterium-dipht

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